Colorectal adenocarcinomas account for the third leading cause of cancer deaths, especially in the Asian countries. The objective of the study is dichoromethane extract of Vanda tessellate was then subjected to qualitative phytochemical analysis to identify the phytoconstituents. Various fractions from the active extract were isolated using the HPTLC technique. High-Performance Thin Layer Chromatography (HPTLC) is a sophisticated and automated method used to characterize its advanced separation efficiencies and detection limits. The dichloromethane extract of Vanda tessellata was further investigated for its mechanism of cell growth. The extract was found to contain steroidal molecule/s and glycoside/s. This indicates that the constituents were partitioned into distinct fractions according to their chemical properties using the HPTLC method. Some fractions were cytotoxic to the adenocarcinoma cell lines. It caused an arrest in the cell cycle in the G0/G1 phase of cell growth in both the adenocarcinoma cell lines. Although the study’s findings are encouraging, additional research is required to ascertain the precise mechanism by which the plant exerts its anticancer properties and to assess its viability as a therapeutic intervention.